Gloria Jean, 92. American soprano and actress was a popular teen performer in the ‘40s, starring in films with Bing Crosby and W.C. Fields. Aug. 31.

For the 75th consecutive year, show attendees can talk face-to-face with hundreds of local companies all under one roof and this year, will have access to some live, on-site design consults for immediate feedback and advice. From simple DIY tasks to total room revamps, homeowners will find everything they need at this year’s Home Show.

Friston isn’t just one of the most influential scholars in his field; he’s also among the most prolific in any discipline. He is 59 years old, works every night and weekend, and has published more than 1,000 academic papers since the turn of the millennium. In 2017 alone, he was a lead or coauthor of 85 publications3—which amounts to approximately one every four days.

The Teijin Group announces that it will showcase a wide variety of high-performance composite products at JEC World 2019.

“The result is that without adding any costs we were able to dramatically reduce the time to complete the project,” Escher said. “This was very important because whenever we took a riser out of service we had about 20 condos that did not have heating or cooling until the new Aquatherm piping and WSHPs were installed and operational.”    

Community contributions don’t stop with just jobs and engines, though. GM foundation grants gave back $75,000 to nine local charities in 2017. The overall economic impact of the plant is staggering, with $143,915,740.23 in-state wages being paid out to employees last year. Furthermore, the plant was the 2017 recipient of Buffalo Business First’s Community Service Award and the 2018 recipient of Buffalo Urban League’s Community Service Award.

No matter how many wind turbines we have in Alberta, when the wind is not blowing it will make precious little difference. 1,445 MW wind capacity X zero wind = zero watts 8,000 MW wind capacity X zero wind = zero watts

The directorship is currently held by William (Billy) Fleming, whose work is focused on the intersection of science, politics and design as they pertain to climate change. Dr. Fleming is co-author of The Indivisible Guide—a progressive, grassroots organization with more than 6,000 groups—and co-creator of Data Refuge, an international consortium of scientists, librarians and programmers working to preserve vital environmental data at risk of erasure during the Trump administration.

29mar8:00 pm11:00 pmHEATHERS THE MUSIC REDOOne Night Special Presentation8:00 pm – 11:00 pm American Repertory Theater of WNY

Only my 1948 farm Jeep has drained me like this project has so far, and trying to get this thing done in time may cause all sorts of issues in the non-wrenching parts of my life, which, at this point, no longer exist. But that doesn’t mean my send it-o-meter isn’t pegged to max, because it totally is.

To determine the composition of the CRL4 complexes that are associated with Vpr and Vpx, we transfected HEK293T cells with expression vectors encoding FLAG-epitope-tagged versions of HIV-1 Vpr, SIVmac239 Vpr, or SIVmac239 Vpx. Twenty-four hours after transfection, the cells were lysed, and epitope-tagged proteins were isolated with FLAG-specific antibody conjugated to agarose beads. The antibody-bound proteins were eluted by competition with FLAG peptide. The eluate was analyzed for endogenous CRL4 complex components by Western blotting with the corresponding antisera. We isolated CRL4 complexes with all three viral proteins as well as their respective cellular targets (Fig. 5).

Intracellular CUL4A and CUL4B distributions vary by cell type.The interchangeability of CUL4A and CUL4B for Vpr and Vpx phenotypes prompted us to examine the intracellular distribution of CRL4 components. Cytoplasmic and nuclear fractions were isolated and probed for CRL4 constituents. In HEK293T cells, CRL4 complex components, including CUL4B, were abundant in the cytoplasm (Fig. 7A, left). However, HEK293T cells are not natural targets of HIV infection. We thus determined whether primary T cells exhibit a similar distribution of CRL4 components. Primary human T cells displayed a CRL4 component distribution profile similar to that of HEK293T cells, with both CUL4A and CUL4B being abundant in the cytosol (Fig. 7A, middle). Interestingly, the CUL4 detected in the nucleus consisted of a single higher-molecular-weight band. This was true for both CUL4A and -B. We have previously shown this higher-molecular-weight band to be the neddylated form of CUL4 (91). Neddylation is required to activate cullin ubiquitin ligases and is also necessary for Vpr and Vpx functions through CRL4 (91, 94, 95). To test whether the single, slower-migrating band detected in the nucleus is indeed neddylated CUL4, we treated HEK293T cells with the neddylation inhibitor MLN4924 before subcellular fractionation. We subsequently probed for CUL4A or CUL4B and, as expected, observed a shift of nuclear CUL4 in the MLN4924-treated cells to a faster-migrating band (Fig. 7B). Thus, nuclear CUL4A and CUL4B are predominately in the active form. In differentiated primary hMDMs, the intracellular distribution of CUL4A and CUL4B differed from that observed for HEK293T cells or primary T cells. In hMDMs, the CUL4 types were more segregated. CUL4A was mostly cytoplasmic, while CUL4B was mostly nuclear (Fig. 7A, right). In HEK293T cells, primary T cells, and primary hMDMs, SAMHD1 was abundant in the cytoplasm. Nuclear SAMHD1 was most readily detected in hMDMs. Of note, UNG2 was not detectable in hMDMs by Western blotting. This is consistent with the terminally differentiated, noncycling nature of these cells (Fig. 7C) (96, 97). These data indicate that, depending on the cell type or proliferation status, the two CUL4 types are not necessarily segregated into separate compartments.