With the sleeve in place, my Jeep XJ-loving friend Michael set about cutting a patch panel to replace the outside wall that was missing from that second, roughly 11-inch long section of frame. To do that, he snagged a piece of cardboard and some scissors, and made this template:

The thing feels rock solid. Though I’ve jumped up and down on the front bumper as hard as I could as an initial check, I’m going to be doing some real validation testing in some empty parking lots and on some empty back roads as soon as I find an un-cracked cylinder head for my AMC inline-six engine. I will hit the worst pothole-ridden streets Michigan has to offer at top speed, which in this vehicle will be about 50 mph, if I had to guess.

Needless to say, this photograph isn’t telling us the whole story. It’s doubtful the entire iceberg is perfectly geometric throughout. But who cares—this photo is an instant classic.

Last June I presented a paper at the International conference in Physics, Computing and Mathematical Modelling (http://www.dpi-proceedings.com/

They include a 3-acre dog park, Bluff Creek Nature Center and Sand Creek Regional Greenway, a 14-mile stretch that connects to Denver’s 800-mile regional trail system. The neighborhood is also adjacent to the Rocky Mountain Arsenal Wildlife Refuge.

For soloists, I’d bet it takes about 30 minutes to set up; for groups, an hour. A bear-hang kit weighs less than a hard-sided canister or an Ursack, but the savings is entirely negated by its inefficiency.

THE ART OF THE BRICK, presented by M&T Bank, at the Buffalo Museum of Science will be on display from February 9 through May 4, 2019, and presale tickets will be available just in time for the holidays on December 1, 2018.

March 21st, 22nd, 23rd March 27th, 28th, 31st (no shows on the 29th & 30th) April 4th, 5th, 6th April 11th, 12th, 13th

Mondaq shall use its reasonable endeavours to make the Website and Services available to you at all times, but we cannot guarantee an uninterrupted and fault free service.

You may also register at www.flickergifts.com Add “product” to your cart, fill out contact info and choose “pick-up in person” for shipping option.

HIV-1 Vpr can trigger G2 cell cycle arrest in the absence of either CUL4A or CUL4B but not both.In order to test whether HIV-1 Vpr specifically requires CUL4A, CUL4B, or both together to trigger G2 cell cycle arrest, we generated HEK293T cell lines that stably express shRNA directed against either CUL4A or CUL4B. These cell lines replicate with kinetics equivalent to those of parental populations but produce little or no detectable CUL4 corresponding to the targeted species (Fig. 1A). HEK293T cells stably expressing shRNA directed against firefly luciferase served as our nontargeting control because firefly luciferase is not expressed in mammalian cells. HEK293T cells are an established cell line for studying HIV replication. Although HEK293T cells lack the CD4 receptor necessary for HIV entry, pseudotyping of the virus with the glycoprotein of vesicular stomatitis virus (VSV-G) enables efficient entry into these cells (74). VSV-G-pseudotyped HIV replicates well in permissive cell lines such as HEK293T cells (75). HEK293T cells, whether depleted of CUL4A or CUL4B, were arrested in response to infection with HIV-1 expressing Vpr but not after infection with a virus lacking the capacity to express Vpr (Fig. 1B and C). To demonstrate the requirement for at least one CUL4 type, we simultaneously depleted both CUL4A and CUL4B and monitored HIV-1 Vpr-mediated G2 cell cycle arrest. Here we transduced HEK293T cells that stably express CUL4A-specific shRNA with a lentiviral vector encoding CUL4B-specific shRNA. To ensure that our results were due to a reduction of CUL4B levels and not due to either lentiviral vector transduction or transient shRNA expression, we transduced another set of cultures with a lentiviral vector encoding the same CUL4A-specific shRNA. This procedure partially eliminated the residual CUL4 type (Fig. 1D). As expected, depletion of both CUL4A and CUL4B reduced HIV-1 Vpr-mediated G2 cell cycle arrest (Fig. 1E and F). Importantly, the reduction in G2 arrest was proportional to the depletion of the remaining CUL4 type.

The marketing and distribution of single use sterile products to the health service sectors and private hospitals.