Roy Clark, 85. The country star and guitar virtuoso who headlined the cornpone TV show “Hee Haw” for nearly a quarter century and was known for such hits as “Yesterday, When I Was Young” and “I Never Picked Cotton.” Nov. 15.

Cell culture.HEK293T cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% fetal bovine serum, 1 mM glutamine, 50 units/ml penicillin, and 50 μg/ml streptomycin. HEK293T stable lines were cultured in the same medium supplemented with 3 μg/ml puromycin.

He said that, in his experience, an industrial robot cannot cut all the way around the tube or pipe uninterrupted. “They can only cut three sides and then they have to stop to finish. What that does is, you get a discontinuity between the cuts, and you cannot get a perfectly clean cut on the end of the tube. It can be up to a quarter of an inch off.”

Stuntman Seriously Injured when Trampled by a Horse Employer: Warstein Limited, Simi Valley At 3:00 p.m. on February 9, 2012, Employee #1 was involved in a movie production at the Big Sky Ranch located in Simi Valley, California. Employee #1 sustained serious injuries (unspecified fractures) when he fell from a horse during movie production and was trampled by the horse. Employee #1 was transported to the Los Robles Medical Center and was hospitalized for seven days. Employee #1 underwent multiple surgeries for his injuries. At 3:00 p.m. on February 9, 2012, Employee #1 was involved in a movie production at the Big Sky Ranch located in Simi Valley, California. Employee #1 sustained serious injuries (unspecified fractures) when he fell from a horse during movie production and was trampled by the horse. Employee #1 was transported to the Los Robles Medical Center and was hospitalized for seven days. Employee #1 underwent multiple surgeries for his injuries.

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As we said in our comments, we expect our valve business to be 38% of the mix of revenue in 2019 and we expect it to be growing in 2020. So that naturally has a positive impact on the margin overall. We did 20% adjusted gross margins in the second half of 2018. We’re guiding to the 19.7%, 19.9% as a good starting point, even with that little bit of deflation in line pipe is our guidance for this year.

Grateful Dead Hour host David Gans proclaimed, “One of the things I love about the Rumpke Mountain Boys is that there’s a fundamental honesty in their presentation. This is something they share with my other heroes, Donna the Buffalo and the Grateful Dead. They don’t make set lists ahead of time, they don’t rehearse their songs to a fare-thee-well– they perform in real time. All four of them write, which is very important, and they draw songs from a tremendous variety of sources… being a musician is a life long learning experience, if you’re doing it right, and they are.”

Can someone please explain to me why our government’s scientific advisers ignore this science? Why they have missed our renewables’ dismal performance, shown in the AESO website? Why do they keep flooding us with misinformation tweets? Who hired them and what “due diligence” did the minister show during their hiring?

12 On a recent Saturday, a man came to the door asking if Friston’s wife was home. When Friston said yes, the man said, “Good, because I got a dead cat here.” He wanted it stuffed.

But Nautilus has been beset by delays which have left its newly-built vessel languishing in a Chinese boatyard, following the inability of its contractor to pay the shipmaker.

Music & Lyrics by Ryan Scott Oliver Based on Photographs by Matthew Murphy Vocal Arrangements & Orchestrations by Ryan Scott Oliver Additional Percussion Arrangements by Jeremy Yaddaw Additional Guitar Arrangements by Matt Hinkley

Addressing the statute of limitations, the Seventh Circuit noted that plaintiffs did not contest that their claims were now outside of the relevant limitations period. The original claim was filed in 2008 and the longest period from any state antitrust statute – six years – barred any claim filed after 2014. Accordingly, the IPPs relied on tolling or relation back arguments. To relate back, plaintiffs needed to show that their claim, under Federal Rule of Civil Procedure 15, "arose out of the conduct, transaction, or occurrence set out—or attempted to be set out—in the original pleading."6 Where the IPPs’ original complaint focused on purchases of steel pipes, tubing, and sheet from distributors and resellers, their new complaint included dozens of household and commercial products making virtually every American consumer and business a putative class member. Plaintiffs argued that their original product definition included "any consumer steel product" but the Court found that the IPPs’ original complaint was limited to products manufactured at steel mills, rather than a host of downstream consumer and industrial products where steel was merely an input. Thus, the Court determined the original definition was insufficient to put defendants on notice of this wider claim. That the IPPs had issued thirdparty subpoenas to consumer product sellers Whirlpool and John Deere was similarly insufficient to put defendants on notice that the IPP claims encompassed all these manufactured end products. While defendants complained about this overbroad discovery in their original motion to dismiss, the Court was not persuaded that defendants had ever been properly notified or – as IPPs suggested – were aware of this broader claim "all along."7 Thus, relation back was unavailable under Rule 15.