WORKING WITH MY SCHOOL AROUND PROBLEM BEHAVIOR PART 1 – WEBINAR March 23 @ 9:30 am – 10:30 am Reserve your spot today: info@parentnetworkwny.org 716-332-4170 www.parentnetworkwny.org/events Once you register, you will

Vpx can initiate SAMHD1 depletion through either CUL4A- or CUL4B-containing CRL4 complexes, but both CUL4 types are required for maximal SAMHD1 depletion in primary macrophages. (A) HEK293T cells that stably express a control shRNA or shRNAs specific for CUL4A or CUL4B mRNAs were either mock infected or infected at equivalent MOIs with HIV-1, HIV-1 lacking Vpr, HIV-2, or HIV-2 lacking Vpr, Vpx, or both. Twenty-four hours after infection, the cells were collected and lysed. Proteins from the whole-cell lysates were resolved by SDS-PAGE and identified by Western blotting using protein-specific antibodies. (B) Cultures of primary human monocyte-derived macrophages (hMDMs) were either mock transfected or transfected with nontargeting siRNA or siRNA specific for the mRNA of the CRL4 complex components indicated. Protein depletion was confirmed by Western blotting. (C, top) Forty-eight hours after siRNA transfection, the cells were either mock infected or infected at a high MOI (MOI = 10) with HIV-2 or HIV-2 lacking Vpx. Less than 24 h after infection, the cells were harvested and lysed. Proteins from whole-cell lysates were resolved by SDS-PAGE and identified by Western blotting with protein-specific antibodies. (Bottom) Densitometric measurements performed on the SAMHD1 bands were normalized to the values of the respective β-actin bands. The relative intensities of the SAMHD1 bands were graphed based on the values obtained from the densitometric analysis. Data shown are representative of a trend observed for samples from 3 different donors.

Reservations are highly encouraged – call (716) 810-9489 to make yours today. Tickets are $45 per person, which includes the show, a 3-course dinner, tax, and gratuity. Cash bar.

Thank you, for joining us today and for your interest in MRC Global. We look forward to having you join us on our first quarter conference call in May. Have a good day, and goodbye.

Our Permian Basin upstream revenue in 2018 was up 62% over 2017. We also ended 2018 in a strong balance sheet position, further reducing our leverage, defined as net debt to adjusted EBITDA to 2.3 times from 2.7 times at the end of 2017.

18mar8:00 pm11:00 pmComedy Open Mic at Tudor LoungeTHE DARKEST COMEDY IN BUFFALO!8:00 pm – 11:00 pm Tudor Lounge

Not at all. Just addressing that “Alberta Advantage” so many people are desperately clinging to, that’s now morphed into the ultimate “Alberta Corporate Advantage.”

Un-Jin Paik Zimmerman, professor emeritus of physiology at the University of Pennyslvania’s Perelman School of Medicine, died October 2 at her home in Bryn Mawr after several months of hospice care. She was 85.

Exposure to Toxic Fumes on Paramount’s Contagion Employer: Paramount Pictures, Los Angeles On November 1, 2010, Employee #1, a foreman sign writer, was working in the front room of the sign shop, gluing double stack central PVC stand-off letters for a production called “Contagion”. Employee #1 used 3 tubes of Devcon Plastic Welder Adhesive and Activator to attach the letters. The Devcon Plastic Welder Adhesive and Activator contain the following: methacrylic acid; methyl methacrylate monomer; chlorosulfonated polyethylene; carbon tetrachloride; 3, 5- diethyl-1, 2-dihydro-1-phenyl-2-propylpyridine; and trade secret ingredients. He stated that he used the product for a total of 2.5 hours and experienced no health symptoms during the use of the product. He stated that later that night, at approximately 9:00 p.m., he started experiencing a headache, which he characterized as more severe than the headaches he normally experiences and also experienced a bloody nose. Employee #1 called in sick during the week as his symptoms became worse. On Friday November 5, 2010, Employee #1 went to the emergency room where he was given pain medication for his headache and was released. On Monday, November 8, 2010, Employee #1 went to his private physician who performed blood tests and diagnosed him as dehydrated with elevated liver enzymes. He was admitted and received an IV for 4 days without any other treatment. Employee #1 was released Friday, November 12, 2010 with no residual symptoms and was informed by his physician that he could return to full duty without work restrictions.

Theatre in the Mist presents the You Can’t Take It With You on Friday, March 29 through April 7 at the Stella Niagara Education Park 4421 Lower River Road, Stella

A group of writers in the 1950’s hysterically compete for the attention of Max Prince during his ongoing battles with NBC.

Got it. Okay. Well, just one more, if I could. In terms of the profitability for first quarter, Jim, you kind of talked about some of the puts and takes with the margin guidance. Should we be thinking about the first quarter gross margin within that range that you guided for the year? Or does it kind of take over the course of the year to wind down to get to that average, so we’re still sort of running at this 20-type-percent level here in the first quarter?